Journal: bioRxiv

Article Title: A critical role for miR-142 in alveolar epithelial lineage formation

doi: 10.1101/253229

Figure Lengend Snippet: (A) Bright field pictures, H & E staining of Control and miR-142 KO lungs at E18.5. (B) IF and WB analysis for Sftpc expression and corresponding quantification of the number of Sftpc-positive cells. (C) Flow cytometry of Control and miR-142 KO lungs for total Epcam, bipotent, AT1 and AT2 cells. (D) Pdpn/Sftpc/DAPI IF staining in Control and KO E18.5 lungs. (E) AT1 and AT2 gene signature analysis in FACS-isolated AT2 cells from E18.5 miR-142 KO lungs. Immunofluorescence for (F) Apc ( G ) Ep300 (H) activated beta-catenin (p S522 Ctnnb1) (I) p-Erk/Cdh1. Scale bar low mag: 100μm, high mag: 25μm.

Article Snippet: For immunofluorescence staining the slides were de-paraffinized, blocked with 3% bovine serum albumin (BSA) and 0.4% Triton X-100 (in Tris-buffered saline (TBS) at room temperature (RT) for 1 hour and then incubated with primary antibodies against Apc (ab15270, Abcam; 1:250), phospho-S 552 -b-catenin (9566, Cell Signaling; 1:250), p300 (sc-585, Santa Cruz; 1:250) and p-Erk (4376, Cell Signaling; 1:250), Sftpc (AB3786, Millipore; 1:500), Cdh1 (610181, BD Trans.Lab; 1:250), Fgfr2-BEK (sc-122, SantaCruz; 1:250), Pdpn (8.1.1, DSHB; 1:250) at 4°C overnight.

Techniques: Staining, Control, Expressing, Flow Cytometry, Isolation, Immunofluorescence

Journal: PLOS ONE

Article Title: Helicase-like transcription factor (HLTF) -deleted CDX/TME model of colorectal cancer increased transcription of oxidative phosphorylation genes and diverted glycolysis to boost S-glutathionylation in lymphatic intravascular metastatic niches

doi: 10.1371/journal.pone.0291023

Figure Lengend Snippet: Source, application and concentration of antibodies.

Article Snippet: Rabbit polyclonal HLTF antibody (NBP1-83256) Novus Biologicals (1:100) Rabbit polyclonal CYTB (55090-1-AP) ThermoFisher Scientific (1:50) Rabbit polyclonal PGAM1 (16126-1-AP) ThermoFisher Scientific (1:50) Rabbit polyclonal ANXA1 (71–3400) ThermoFisher Scientific (1:20) Rabbit monoclonal γH2AX-phospho S139 (ab81299) Abcam (1:50) Rabbit monoclonal ENO1 (ab227978) Abcam (1:2,000) Rabbit recombinant monoclonal mouse-specific PDPN (MA5-29742) ThermoFisher Scientific (1:500) , IHC-P: Vector Laboratories RTU Biotinylated Goat Anti-rabbit IgG (H+L) (BP-9100-50).

Techniques: Concentration Assay, Recombinant, Plasmid Preparation

Journal: Cancer Science

Article Title: Impact of Vitamin C on Immune Response and Edema Following Near‐Infrared Photoimmunotherapy ( NIR‐ PIT )

doi: 10.1111/cas.70070

Figure Lengend Snippet: In vitro near‐infrared photoimmunotherapy (NIR‐PIT) using cetuximab‐IR700 (Cet‐IR700) in A431‐GFP‐luc cells and podoplanin‐IR700 (PDPN‐IR700) in MOC1 cells. (A) Detection of Cet‐IR700 bound to A431‐GFP‐luc cells and PDPN‐IR700 bound to MOC1 cells by flow cytometry. (B) Cell viability after Cet‐IR700 NIR‐PIT (Cet‐PIT) in A431‐GFP‐luc cells and PDPN‐IR700 NIR‐PIT (PDPN‐PIT) in MOC1 cells in vitro measured by MTT assay ( n = 5, mean ± SEM, one‐way ANOVA, followed by Dunnett's test). (C) Cell viability of A431‐GFP‐luc and MOC1 cells after NIR‐PIT with L‐NaAA was measured using MTT assay ( n = 5, mean ± SEM, one‐way ANOVA, followed by Dunnett's test). * p < 0.05; *** p < 0.001; **** p < 0.0001 versus NIR‐PIT without L‐NaAA.

Article Snippet: Anti‐mouse PDPN antibody (clone 8.1.1) and anti‐mouse CD25 antibody (clone PC‐61.5.3) were purchased from Bio X Cell (Lebanon, NH, USA).

Techniques: In Vitro, Flow Cytometry, MTT Assay

Journal: Cancer Science

Article Title: Impact of Vitamin C on Immune Response and Edema Following Near‐Infrared Photoimmunotherapy ( NIR‐ PIT )

doi: 10.1111/cas.70070

Figure Lengend Snippet: Efficacy of in vivo PDPN‐targeted NIR‐PIT in MOC1 tumors with or without L‐NaAA. (A) Treatment schedule. (B, C) Immune cell populations in the TDLNs analyzed by flow cytometry 1 and 2 days after NIR‐PIT. (B) The expression of CD86 on DCs in the TDLNs ( n = 5, mean ± SEM, one‐way ANOVA, followed by Tukey's test). (C) The expression of CD69 and CD25 on CD8+ T cell in the TDLNs 2 and 4 days after NIR‐PIT ( n = 5, mean ± SEM, one‐way ANOVA, followed by Tukey's test). (D) Representative pictures of MOC1 tumor sections 6 days after NIR‐PIT (images; ×200; scale bar, 20 μm). Staining for CD8, GZMB, and pan‐cytokeratin (pCK) is shown in magenta, green, and cyan, respectively. The insets (a–c) are enlarged and displayed on the right side. They show representative images of a CD8+GZMB− cell in control group (a), CD8+GZMB+ cell in NIR‐PIT without L‐NaAA group (b), CD8+GZMB+ cell in NIR‐PIT with L‐NaAA group (c). (E) CD8+ T cell density (left) and CD8+ and GZMB+ T cell density (right) in the tumor area ( n = 4, mean ± SEM, one‐way ANOVA followed by Tukey's test). (F) CD8+ T cell density (left) and CD8+ and GZMB+ T cell density (right) in the stroma area ( n = 4, mean ± SEM, one‐way ANOVA followed by Tukey's test). (G) Tumor volume curves in MOC1 tumors ( n = 6, mean ± SEM, repeated measures, two‐way ANOVA followed by Tukey's test). (H) Survival curves ( n = 6, log‐rank test with Bonferroni correction). * p < 0.05; ** p < 0.01; *** p < 0.001; ns, not significant versus control.

Article Snippet: Anti‐mouse PDPN antibody (clone 8.1.1) and anti‐mouse CD25 antibody (clone PC‐61.5.3) were purchased from Bio X Cell (Lebanon, NH, USA).

Techniques: In Vivo, Flow Cytometry, Expressing, Staining, Control